Journal: Pathophysiology
Article Title: The Effect of TGFβ1 in Adipocyte on Inflammatory and Fibrotic Markers at Different Stages of Adipocyte Differentiation
doi: 10.3390/pathophysiology29040050
Figure Lengend Snippet: Effect of TGFβ1 on Collagen VI protein, ( A ) Commitment stage of adipocyte differentiation: The result is a representative result of 2 independent experiments. Data expressed as Mean ± SD. One-way ANOVA with Tukey’s multiple comparison test was used to compare the effect of TGFβ1 on Un-Diff and R-Diff. p -value < 0.05 * vs. Un-Diff, vs. R-Diff. ( B ) Mature adipocytes: The result is a representative result of 3 independent experiments. Data expressed as Mean ± SD. One-way ANOVA with Tukey’s multiple comparison test was used to compare Un-Diff, S-Diff, and R-Diff. Two-way ANOVA with Sidak’s multiple comparison test was used to compare the effect of TGFβ1 on S-Diff and R-Diff. p -value < 0.05 * vs. Un-Diff, vs. S-Diff. vs. R-Diff, vs. S-Diff + TGFβ1. The levels of collagen VI protein, visualized by Western blot, were normalized to total protein loading obtained from stain-free imaging and shown in the histogram.
Article Snippet: In order to study the effect of the TGFβ1 on different phases of adipocyte differentiation (preadipocyte, commitment, and terminal differentiation), recombinant active human (rh) TGFβ1 protein (2 ng/mL: Catalogue Number 240-B-010, R & D Systems, Minneapolis, MN, USA) was used.
Techniques: Comparison, Western Blot, Staining, Imaging